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Before immunoprecipitation, samples containing equal amounts of proteins were precleared with protein A or protein G agarose/sepharose (Millipore) and subsequently incubated with various irrelevant IgG or specific antibodies in the presence of protein A or G agarose/sepharose beads, as described previously (Hsu & Youle, 1998

In an Alzheimers disease mouse model, FPN gene deletion increased ferroptosis, leading to memory impairment, whereas restoring FPN improved ferroptosis and memory deficits (Bao et al., 2021)

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Briefly, after treatments supernatant was recollected and centrifuged at 300 g at 4 C for 10 min to eliminate cell debris