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After removing the final wash, the beads were incubated twice at room temperature in 80 l of the digestion buffer (2 M urea, 50 mM Tris-HCl, 1 mM DTT and 0.4 g of trypsin) while shaking at 1,000 rpm

Pretreatment with JA for alkaline stressed plants mitigated the toxic effects of Na 2 CO 3 and improved the Chl content by 41.26 and 40.11% and carotenoids by 32.43 and 24.00% in 100 mM Na 2 CO 3 + 10 M JA and 150 mM Na 2 CO 3 + 10 M JA treatments, respectively, compared with that of 100 mM and 150 mM Na 2 CO 3 treatments, respectively

Ajami M

Int J Mol Sci 16(8):1719317230 Panner Selvam MK, Sengupta P, Agarwal A (2020) Sperm DNA Fragmentation and Male Infertility

However, the complex developmental origin and tissue composition of the IVD along with the avascular nature of the mature disc makes regeneration of the IVD a uniquely challenging task