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Description

5 , e002816 (2016)

N., Uramoto, G

Following differential centrifugation, the P2 microsomal membrane fraction was resuspended in cold buffer (50 mm Tris-HCl, 150 mm NaCl, 12.5 mm MgCl2, 0.2% BSA, and 10% glycerol, pH 7.4), supplemented with protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail, aliquoted, and stored at 80 C until use