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2009 Oct;72(7):2025-32

Acetic Acid (0.6%) or Hydrochloric Acid (HCl 0.1M) Used for peptides that have poor water solubility

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The removal of the staining working solution and subsequent rinsing of the cells with PBS was imperative, pre-warmed PBS was added and the cells were incubated for 5 min and photographed and documented under an inverted fluorescence microscope

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