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Sema4D-induced translocation of AR to the nucleus is dependent on the GAP domain of PlexinB1 To determine whether the GAP domain of PlexinB1 is required for Sema4D-induced nuclear trafficking, PC3 cells were transfected with AR-flag and WT PlexinB1 or a mutant form of PlexinB1 (PlexinB1-RA), which lacks GAP activity [23]