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Pellets were resuspended in DMEM/F-12 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 20% foetal bovine serum (FBS, Pan Biotech, Aidenbach, Germany) and penicillin/streptomycin (Life Technologies) and plated on 10 cm cell-culture dishes

Diabetes Metab 3 , e00116 (2020)

doi: 10.1016/j.mrgentox.2008.11.012

The satellite peaks were modelled using their heteronuclear J-coupling constant ( 1 J CH ) either determined experimentally in vivo (Glx( 13 C)H2 and Lac( 13 C)H3) or estimated from the literature