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To evaluate T cell induction, splenocytes from mice immunized with either the OspA mRNA or recombinant OspA protein vaccine were incubated with a pool of 66 overlapping 15-mer OspA peptides synthesized by GenScript's peptide library services

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Real-time qRT-PCR analyses Total RNA was isolated from WAT (epididymal fat pad, unless stated otherwise) and liver and subjected to qRT-PCR analyses using SYBR Green, according to the manufacturers protocol