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A 5 mm 5 mm mycelial plug was excised and inoculated into a Granny Smith apple, which was then wrapped with cling film, placed in a zip-lock bag, and incubated in the dark at 22C for 5 days ( Phytophthora -selective NARPH agar plates (19.5 g/L PDA, amended with 50 mg/L nystatin, 200 mg/L ampicillin, 100 mg/L pentachloronitrobenzene, 10 mg/L rifampicin and 50 mg/L hymexazol) and incubated at 22C for 2 days in the dark ( 2 PO 4 ( LPV3 fragment following the protocol described in 2 PO 4 and incubated with agitation at 115 rpm in the dark at 22C for 5 days