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1 mL extract was mixed with 1 mL acid ninhydrin [1.25 g ninhydrin (HiMedia, Mumbai, India) dissolved in 30 mL glacial acetic acid (HiMedia, Mumbai, India) and 20 mL ortho-phosphoric acid (HiMedia, Mumbai, India)] and 1 mL glacial acetic acid (SRL, Mumbai, India)

Bi-Est (a combination of estriol and estradiol, typically in an 80:20 ratio) remains the most commonly prescribed compounded estrogen preparation

In addition, using an anti-HPV-16 E7 antibody we immunoprecipitated HPV-16 E7 in the CaSki [20] and SiHa [21] cervical carcinoma cell lines, known to express HPV-16 E7 endogenously [22], [23], GSTP1 also co-precipitated with the viral oncoprotein in these cells, as detected by Western blot analysis (Figure 1F)