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The cells were resuspended in relaxation buffer (100 mM KCl, 3 mM NaCl, 3.5 mM MgCl 2 , 1 mM ATP and 10 mM PIPES pH 7.3) containing protease inhibitors (cOmplete EDTA-free, Roche)

Best injection sites: the abdominal wall (two inches from the navel) and the outer thigh are the two most reliable sites for self-administration

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However, there is tremendous variation in cellular responses to SARS-CoV-2, depending on factors including the cell type, organ type, metabolic and physiological context, patient genetics, individual clinical characteristics (e.g., age, sex, comorbidities), and stage/severity in the COVID-19 disease