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The main causes of fatigue are: accumulation of protons in the muscle cell, depletion of energy sources (e.g., phosphocreatine and glycogen), accumulation of ammonia in the blood and tissues [2,3,4], oxidative stress, muscle damage [1] and changes in neurotransmitter synthesis, such as the increase in serotonin and the decrease in dopamine [5]

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doi: 10.1016/j.nutres.2023.08.010 93

Summary Keywords traditional Chinese medicine, active ingredient, ferroptosis, non-alcoholic fatty liver disease, protective effect Citation Wu Q, Chen Z, Ding Y, Tang Y and Cheng Y (2022) Protective effect of traditional Chinese medicine on non-alcoholic fatty liver disease and liver cancer by targeting ferroptosis

The slides were pre-coated with 150 L of 0.5% normal-melting agarose in PBS, and were specially designed for this assay