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Future research should differentiate between symptomatic and asymptomatic infected individuals, select specific probiotics or their fermented products, utilize widely studied strains, standardize experimental protocols (including dosage, duration, and clinical endpoints), and assess the long-term effects of probiotic interventions

and (3) the lower plasma zinc may be due to a combination of the above two factors

Caillon A, Paradis P, Schiffrin EL

( A , B ) Myocardial infarction (one isoprenaline challenge) and re-infarction (two isopre- naline subsequent challenges) in isoprenaline- treated rats (75 mg/kg sc (gray bars), 150 mg/kg sc (white bars))

So to avoid culture dieing, subculturing is very important