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In fact, there is an added layer of complexity in how S-nitrosation controls cellular responses, as some proteins are S-nitrosated basally, and loss of this modification (hypo-nitrosation) results in pathologic effects whereas other are not S-nitrosated to begin with and modification of critical thiols (hyper-nitrosation) has deleterious results

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For example, a patient with decreased levels of a polypeptide receives a daily dose 0.1-100 ug/kg of the polypeptide for six consecutive days

Then, all wells were thoroughly resuspended and plated for CFUs on LB agar to assess the number of surviving cells