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Previously, a conspicuous relationship was observed between purine and monoamine metabolite concentrations in cerebrospinal fluid (CSF) during depressive illness suggesting the presence of a parallel purinergic and monoaminergic activation in the brain (Niklasson et al., 1983)

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The worms were then washed twice with 1 PBS and mounted onto 2% agarose pads for visualization using a ZEISS Axio Observer.Z1 (ZEISS, Oberkochen, Germany) inverted microscope equipped with a ZEISS AxioCam MRc color camera and a ZEISS Plan-Apochromat 20/0.8 WD 0.55 mm air immersion objective