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Bagchi, S., Yuan, R

The resulting suspension should be sonicated with an ultrasonic cell disrupter or subjected to 2 freeze/thaw cycles to further break the cell membranes

10.1016/j.biomaterials.2014.06.017 214 PereraK.IvoneR.NatekinE.WilgaC

Without this preservative, a reconstituted peptide solution would be vulnerable to bacterial contamination, which would quickly degrade the peptide and compromise your research