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Data were collected using the shutterless collection mode

Samples were purified by SPE before analysis of eicosanoids by LCMS/MS, which was previously described in the methodology on endocannabinoids

This ELISA kit only detects the free form of human IGF1, it does not detect complexed or bound forms such as IGFBP-bound IGF1

The combination protocol preserves insulin sensitivity and allows natural GH troughs that promote fat oxidation, while MK-677's sustained elevation antagonizes insulin signaling and reduces fasting lipid oxidation rates

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