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Supernatants were then collected and the serum levels of AST, ALT, ALP, triglyceride (TG), cholesterol (CHO), high-density lipoproteins (HDL), and low-density lipoproteins (LDL) were determined using commercially available reagents (Roche-Diagnostics, Indianapolis, IN, USA) in accordance with the standard operating procedures recommended by the manufacturers

Overexpressed IGFBP-1 in transgenic mice shows that its increase produces hyperinsulinaemia accompanied by glucose intolerance, and that this process is dependent upon IGFBP-1 phosphorylation statewhich is increased in diabetes [259]

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