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At the protein level, circadian oscillation of clockwork negative factors such as PERs and CRYs were expected to be in anti-phase with BMAL1, and to be more highly expressed during the dark period relative to the night based on studies on mouse SCN (36)

However, whereas the pharmacotherapy literature in cirrhosis has focused primarily on safety, the challenge with non-pharmacologic modalities is the near-absence of efficacy data specific to this population

pDNA concentration and purity were measured on a NanoDrop One (Thermo Fisher, UK), and then linearized using MluI for 3 h at 37 C, followed by heat inactivation at 80 C for 20 min