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Branched-chain amino acids as biomarkers in diabetes

Cell culture, retroviral transduction, and NGFR-LMP1 activation Wild-type MEFs and derivatives thereof, TRAF6 -/- MEFs 79 , and HeLa cells (obtained from the German Collection of Microorganisms and Cell Cultures, GCMC) were kept in Dulbeccos modified Eagles medium (DMEM, Thermo Fischer, #41966-029)

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This adaptive response may be caused by mutations that deactivate or activate tumor suppressor genes or oncogenes under hypoxic conditions

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