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People exposed to nitrous oxide (medical or recreational use) may develop functional B12 deficiency, as the gas inactivates B12-dependent enzymes, potentially requiring supplementation

The STEP trial extension data covers up to 2 years, showing maintained weight loss as long as treatment continues

Therapeutic doses of vitamin D can differ based on the same variables, but 50,000 IU (1,250 mcg) is a common amount according to the journal Nutrients

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But it failed to demonstrate significant weight loss in six clinical trials, and its development was terminated because of those failures