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When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature

In a 96-well plate, the adduct solution was treated with the appropriate amount of an aqueous stock solution of GSH, GSSG, cysteine, H 2 S or taurine, and the solutions were incubated at 20 C for 3060 min

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4, the exposure of hepatocytes to both chemicals resulted in a significant increase in intracellular glutathionylated protein levels (Fig