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3) While more than 170 chemical components have been identified in ASA, the pharmacological evaluation has been limited to a few compounds such as amygdalin, its metabolites, total polyphenols, and total volatile oils

Briefly, cells were treated with 100 M BSO (dissolved in culture medium) for 18 h and washed with fresh serum free DMEM followed by aspirin treatment as above

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Molecular graphics were generated using Pymol 62 and UCSF ChimeraX 63

To address this question, we mutated Tyr110 in the CPYS-motif of ScGrx7 as a candidate residue that might contribute to the glutathione activator site (see Discussion for details) and performed a preliminary study with wild-type ScGrx7 as a control