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Repeated dosing studies in rats, dogs, and mice for up to 6 weeks across doses from 0.006 mg/kg to 20 mg/kg via multiple routes showed excellent tolerance

Oxidative stress, assessed via the GSH/GSSG ratio, increased in both HepG2 and HepG2 ATP7B-KO cells across all copper doses

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Under these circumstances, Nrf2 migrates to the nucleus and activates genes driven by the antioxidant response element (ARE), thereby promoting redox homeostasis