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Flow cytometric analysis revealed that although intranasal IL-33 induced robust eosinophilia in both lung and BALF, melatonin significantly limited this increase ( Figures 2G, H ), even under melatonin receptor antagonism ( Figures 2G, H )

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Simply put, it is to insert cysteine residues at specific points of the antibody through genetic engineering, and then couple the hydroxyl group on the cysteine with the toxin to form a site-specific antibody-drug conjugate

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[57] Nguyen MM, Ahmann FR, Nagle RB, et al