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For immunohistochemistry (IHC) labeling, heat-mediated epitope retrieval was performed in 0.01 m sodium citrate buffer, pH 6, containing 0.05% Tween-20, which was heated in a microwave oven to 95C for 30 min

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But youll also probably need imaging tests, or even biopsies, before your doctor can make a specific diagnosis

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In comparison to Arabidopsis, however, lost GST subfamilies, such as Theta and Phi, indicating that the Theta and Phi subfamilies were lost during evolution or after divergence in the last common ancestor