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Subsequently, all samples were subjected to centrifugation at 1500 rpm for 15 min at 4 C, facilitating a clear delineation of the two phases: the upper (lipophilic) phase, which contains the apolar metabolites, and the lower (hydrophilic) phase, which comprises the polar metabolites of interest

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Ni is a noxious agent that impacts plant species by altering enzyme function and hindering seed germination and growth (Pandolfini et al., 2006