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Finally, for in vitro experiments, absorbance values were transformed into activity percentages following the next formula: Where A S is the absorbance of the inhibitor sample at a specific concentration, A SB is the blank of the inhibitor sample, A C is the highest absorbance (without inhibitor), and A CB is the blank of the highest absorbance

The polydispersity index (PDI) of the products was 0.126 0.015 and the average size of SLNs was 78.72 0.85 nm

doi: 10.3389/falgy.2025.1608202 Received 08 April 2025 Accepted 11 July 2025 Published 08 August 2025 Volume 6 - 2025 Edited by Nicola Diny, University of Bonn, Germany Reviewed by Daniel Radtke, University Hospital Erlangen and Friedrich-Alexander University Erlangen-Nuremberg, Germany Yash Choksi, Vanderbilt University Medical Center, United States Updates Copyright 2025 Tomii and Kano

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Ghadge V, Kumar P, Singh S, Mathew DE, Bhattacharya S, Nimse SB, Shinde PB (2020) Natural melanin produced by the endophytic Bacillus subtilis 4NP-BL associated with the halophyte Salicornia brachiata