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Thus, whilst we could obtain hits from both the NMR and XChem fragment screens, the cross-platform validation was generally limited, which prevented the rational design of larger, more potent analogues

Protein was transferred to nitrocellulose membrane

It's probably closer to 9 out of 10

Growth factor upregulation (VEGF pathway): Also well-represented in the published literature

In the seizure-operated groups, the cerebrolysin-administered group showed a significant decrease in the number of apoptotic cells in the hippocampal CA1 and CA3 regions compared to the vehicle group