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This assay was performed on MS medium for the convenience of identifying a clear phenotype, which will be difficult on CIM as callus induction and/or growth might interfere with the phenotype

Kobayashi T, Ishida T, Horiike K, Takahara Y, Numao N, Nakazawa A, Nakazawa T, Nozaki M (1995) Overexpression of Pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered Escherichia coli

In line with this observation, Taraseviciene-Stewart et al

Experimental evidence suggests that the expression of GPx-2 and GPx-4 is less sensitive to variations in selenium levels compared to GPx-1 or GPx-3 (see section III)