lipo- c Lipo-Mino-Mix-C enzyme function LIPO C + B12 –

doi: 10.1038/s41580-019-0133-3 30 WangJPanYWeiGMaoHLiuRHeY

Crypts ( N = 500) per well were seeded in 50 l Matrigel (BD, 356231) on a pre-warmed 24-well plate and allowed to solidify for 10 min at 37 C, after which 500 l IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 g/ml streptomycin (Biochrome, 0257F) was added

Libraries were sequenced to an average depth of ~20 M aligned reads for differential gene analyses

Motuzaite-Matuzeviciute et al., 2012)

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