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Dogs with the ACE variant had lower levels of ACE activity even in more advanced mitral valve disease than dogs without the variant." They concluded that the impact of their finding "on the need for ACE-I in dogs with the polymorphism and heart disease deserves further study." Then, in a September 2023 abstract, the same authors' aim was to determine the impact of certain angiotensin converting enzyme (ACE) gene polymorphisms upon the presence of congestive heart failure (CHF) in MVD-affected cavaliers

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Figure 6 To further elucidate whether these alterations in mitochondrial Ca 2+ retention capacity were due to changes in cyclophilin D or MCU, we developed western blots for these two proteins