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Adipocytes were maintained another 48 hours in culture medium followed by a 24 hour incubation with either 1 or 0 mmol/L Mg 2+ (Sigma-Aldrich) DMEM medium (containing 25 mmol/L D-glucose, 0.04 mmol/L Phenol Red, 1 mmol/L sodium pyruvate, 1.8 mmol/L CaCl2, 0.00025 mmol/L Fe(NO3)3, 17.9 mmol/L NaHCO3, 5.3 mmol/L KCl, 110.3 mmol/L NaCl, 0.9 mmol/L NaH2PO4, 0.4 mmol/L glycine, 0.4 mmol/L L-Arginine hydrochloride, 0.2 mmol/L L-Cystine 2HCl, 4 mmol/L L-Glutamine, 0.2 mmol/L L-Histidine hydrochloride, 0.8 mmol/L L-Isoleucine, 0.8 mmol/L L-Leucine, 0.8 mmol/L L-Lysine hydrochloride, 0.2 mmol/L L-Methionine, 0.4 mmol/L L-Phenylalanine, 0.4 mmol/L L-Serine, 0.8 mmol/L L-Threonine, 0.08 mmol/L L-Tryptophan, 0.4 mmol/L L-Tyrosine disodium salt dihydrate, 0.8 mmol/L L-Valine, MEM Vitamin Solution (Thermo Fisher Scientific, Waltham, MA USA) and 10% (v/v) FBS)

10.1002/jcc.20290 32 CavasottoC

Editor information Editors and Affiliations Rights and permissions Copyright information 2016 Springer International Publishing Switzerland About this chapter Cite this chapter Csiszr, J., Horvth, E., Bela, K., Gall,