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All reactions were performed in a 1.5 L volume using the following PCR program: initial denaturation at 95C for 2 min, followed by 45 tri-step cycles of amplification: 10 s at 95C, 10 s at 65C, and 10 s at 72C

NAC is a stable, well-absorbed way to deliver it, which is why it is the cysteine source of choice in supplements

At 5 mg/mL, it equals 50 units

Secondly, it may activate short-chain fatty acid receptors (SCFA receptors) and inhibit histone deacetylase (HDAC) activity, thereby lifting transcriptional repression on key iron absorption genes (such as Dcytb and Ferroportin) and consequently upregulating intestinal iron uptake

2.4 Association between neuronal metabolic reprogramming and neurodegenerative diseases A key area of interest is the interplay between neuronal energy metabolism and neurodegenerative diseases