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Research indicate that inactivity independently increases the risk of skeletal muscle wasting, osteoporosis [62], type 2 diabetes, cardiovascular disease and depression [63]

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Conjugated antibodies were used against mouse CD5 (ref 130102574, FITC-conjugated), PBS57-loaded CD1 d Tetramer (NIH facility, PE-conjugated), NK1.1 (ref 130103963, PerCp-Cy5.5conjugated), CD4 (ref 130102411, PE-Cy7-conjugated), CD25 (ref 130102550, APC-conjugated), CD69 (ref 561238, Alexa700-conjugated), TCR (ref 130104016, APC-Vio770 conjugated), TCR- (ref 130104815, V450-conjugated), CD8 (ref 130109252, V500-conjugated), CD45 (ref BLE103140, BV605-conjugated), I-Ab (ref 130102168, FITC-conjugated), F4/80 (ref 130102422,PE conjugated), CD103 (ref 563637, PerCP-Cy5.5-conjugated), CD11c (ref 558079, PE Cy7-conjugated), CD86 (ref 560581, Alexa-700 conjugated), Ly6G (ref 560600, APC-H7conjugated), CD11b (ref 560455, V45O conjugated), CD45 (ref 130402512, V500 conjugated), Ly6C (ref BLE128036, BV605-conjugated) (BD Biosciences, Franklin Lakes, United States

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