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(5) Cell Washing: Gently wash the upper chamber of the Transwell with PBS to remove non-invaded cells

(Li and Gong, 2022)

CFPAC-1 and SW1990 cell lines were transfected with shVRK2 plasmids, and PANC-1 and BxPC-3 cell lines were transfected with VRK2-overexpressing plasmids by applying Lipofectamine 3000 reagent according to the manufacturers protocol

Several lines of evidence suggest that ROS can contribute both directly and indirectly to killing by causing oxidative damage to various biomolecules or by stimulating pathogen elimination through various non-oxidative mechanisms [106]

Only 5.8 percent of the participants had consumed fish oil supplements in the previous three months and only 3.1 percent consumed fish oil supplements for more than 60 days over the previous three months