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The barrier effect of the BBB is mainly formed by ECs

History of endoscopic treatment of vesicoureteral reflux

Cell culture Caco-2 cells, a human colorectal carcinoma-derived epithelial cell line, were cultivated in Dulbeccos modified Eagles medium (DMEM)/F12 (Gibco) containing 10% fetal bovine serum (FBS) in a humidified incubator at 37 C, 5% CO 2