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For example, expression cloning is employed wherein polyadenylated RNA is prepared from a cell responsive to the polypeptides, for example, NIH3T3 cells which are known to contain multiple receptors for the FGF family proteins, and SC-3 cells, and a cDNA library created from this RNA is divided into pools and used to transfect COS cells or other cells that are not responsive to the polypeptides

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2021;12:618196 Liu S, Peng N, Xie J, Hao Q, Zhang M, Zhang Y, et al

doi: 10.1016/j.ccr.2008.11.012 107 GacheruSNTrackmanPCShahMAOGaraCYSpacciapoliPGreenawayFTet al