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Description

Guide and passenger RNAs were each chemically synthesized (IDT) and gel-purified on a urea-polyacrylamide gel, then annealed into duplexes at 5 M final concentration in annealing buffer (30 mM Tris-HCl pH 7.5, 100 mM NaCl and 1 mM EDTA)

10.1183/09031936.01.17303370 157 SchuligaM.ReadJ.KnightD

Neutrophils' Extracellular trap mechanisms: from physiology to pathology

Types of glaucoma Primary glaucomas show optic neuropathy in the presence or absence of elevated IOP (i.e., the cause of the disease is not known) whereas secondary glaucomas are defined by known pathological mechanisms that elevate IOP above normal