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The cell pellets were vortexed well to ensure complete lysis and centrifuged at 4 C for 10 min at 15,000 rpm

This clearly implies that, although several in vitro tests for genotoxicity assessment are available at different stages of development and acceptance, they cannot be considered to fully replace animal tests needed to evaluate the safety of substances (see Adler et al., 2011)

We chose to make all transport rates linear and adjusted them so that the measured APAP, APAP-S, APAP-G, and NAPQI-GSH concentrations in the plasma and the urine were as measured in the literature[4, 6, 11, 3032]

doi: 10.1126/science.aat0835 53

Additionally, immunostaining with anti-Ly6C revealed a decrease in Ly6C + monocytes within the plaque of P-Mn/TI@mHSA-treated mice (Supplementary Fig