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Initially, dexamethasone (DEX) and ursodeoxycholic acid (UDCA) were co-encapsulated via the solid-in-oil-in-water emulsion solvent extraction-evaporation technique, which employs dichloromethane as the only organic solvent and ethanol as a co-solvent in an 80:20 ratio

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After 3 days, confluent cells were detached using 1 trypsinEDTA solution and reseeded at 2 10 6 cells in 20 ml medium in 175 cm 2 flasks

There is evolving experimental evidence that chronic alcohol abuse inhibits transport-mediated absorption of dietary zinc in the small intestine and, in parallel, decreases zinc transport into the alveolar space and uptake by the alveolar macrophage