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Dopaminergic neurons in the substantia nigra , the brain region most affected by Parkinson's disease, express receptors for an inflammatory cytokine called tumor necrosis factor-alpha (TNF-) , which suggests that excess TNF- released by nearby activated microglia may damage nigral dopaminergic cells

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At the same time, increased ROS causes the mPTP to open, which both causes and results from mPTP opening

To assess glucose metabolism, mice were fasted for 12 h, after which ~3 L of blood was collected from the tail vein, and fasting blood glucose (FBG) was measured using a Roche blood glucose meter (Roche Diagnostic GmbH, Germany)

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