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2.1 From reproductive cycle to menopause The female reproductive lifespan is governed by the hypothalamic-pituitary-ovarian (HPO) axis, which orchestrates cyclical hormonal dynamics essential for ovulation, endometrial remodeling, and fertility (Spaziani et al., 2021)

Moreover, pretreated cells displayed higher basal GCLC levels than cells without pretreatment, and the second NFT treatment led to a relatively modest additional increase (Fig