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littoralis reported, however, that feeding 4MSOB ITC at 2 or 4 mol per g in artificial diet led to a high rate of GSH conjugate formation, which depleted the supply of GSH and Cys, causing up to a one-third reduction in growth 29

Once the mixed population of the specific nucleic acid sequences is generated, the polynucleotides can be used directly or inserted into an appropriate cloning vector, using techniques well-known in,the art

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