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After washing, the target is eluted off and ready for downstream applications

In contrast, in the HT-29 colon cancer cell line, it was determined that metallothioneins can bind up to 10% of Zn(II) (Krel and Maret 2006)

Ishitsuka R, Yamaji-Hasegawa A, Makino A, Hirabayashi Y, Kobayashi T

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Elimination occurs primarily through renal excretion of metabolites, with the peptide and its fragments detectable in urine for up to 4 days following administration despite plasma clearance within hours