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Methods: The project comprised of two phases

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The ELISA kits used were bought from Thermo Fisher, USA, their detailed information is as follows: IL-4 Mouse Uncoated ELISA Kit (#88-7013-88), IL-5 Mouse Uncoated ELISA Kit (#88-7054-88), IL-13 Mouse Uncoated ELISA Kit (#88-7439-88), IL-17A (homodimer) Mouse Uncoated ELISA Kit (#88-7371-88 A), IL-23 Mouse Uncoated ELISA Kit (#88-7230-88), IL-6 Mouse Uncoated ELISA Kit (#88-7066-88), IL-33 Mouse Uncoated ELISA Kit (#88-7333-88) and TNF alpha Mouse Uncoated ELISA Kit (#88-7324-88)