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For systemic or gut-related protocols, abdominal subcutaneous injection is the standard approach

To ensure specificity and accuracy of the amplification process, both positive and negative controls were included: positive controls (PC) consisted of RNA from PCa cell lines (LNCaP, PC3 and VCaP) known to express target genes such as AR and negative controls included RNA from healthy donor PBMCs that do not express tumor-specific genes, along with no-reverse transcription (no RT) and water controls

Reusability Single-use bottles are convenient, but they are not ideal for repeated refilling

differentiation from pseudoseizure pseudoseizure (technical terminology varies, including Paroxysmal Non-Epileptic Seizures or psychogenic, non-epileptic attacks or functional seizures) A clinical phenomenon that mimics a seizure, often related to psychiatric stress

Borgens RB, Liu-Snyder P