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The mood stabilizers lithium and valproate attenuate a series of METH-induced changes, such as reduction of mitochondrial Cyt c, the mitochondrial anti-apoptotic Bcl-2/Bax ratio, activity of mitochondrial Cyt c oxidase, and the expression of several proteins related to the apoptotic pathway

To be noted, one highlight of this study is the experiments on cell identification and enrichment of whole-brain tissue samples, demonstrating their strategy achieved around 90% cell specificity of selective transfection with no extra use of a cell-specific promoter

To evaluate the effects of compound on GPX4 activity, compound was first pre-incubated with purified GPX4 in the assay buffer [Tris-HCl (100 mM, pH 7.4), EDTA (5 mM), Triton X-100 (0.1% v/v), NADPH (0.2 mM), glutathione (3 mM), and glutathione reductase (1 unit)] for 5 min at 37C

doi: 10.3389/fbioe.2021.643722 171 ZhengY.LiuW.QinZ.ChenY.JiangH.WangX

Rebholz, C