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revealed that legumain deficiency alleviated the development of renal tubular injury and ferroptosis in AKI mice, which proved legumain enhanced renal tubular epithelial cell ferroptosis by facilitating autophagy in AKI( Inflammation During ferroptosis, the shrinkage of cell and organelle membranes and the increase of permeability cause cell content release, including damage-associated molecular pattern (DAMPs) (Mu et al., 2021)

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As FDA states in their guidance document on the Q-Submission program, Early interaction with FDA on planned non-clinical and clinical studies and careful consideration of FDAs feedback may improve the quality of subsequent submissions, shorten total review times, and facilitate the development process for new devices. While this is not meant to be a general pre-review of your submission, it is there to help you get answers to questions directly from FDAwhich will contribute to a smooth submission and, hopefully, approval