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While kojic acid soap is generally safe for most skin types, those with sensitive skin should use it cautiously and perform a patch test first

(2002) first developed astrocyte-specific NF1 conditional knockout mice using Cre/loxP technology in which Cre was expressed in astrocytes by E14.5, and the astrocytes in these mice were NF1 null ( GFAPCre

& Iwai, K

Macrophages and white blood cells flood the damaged area, and the resulting tissue fluid exudation leads to erythema with a radius of 3 to 5 cm on the skin surface

A suggested strategy is to use 2D cultures for rapid initial screening to identify relevant effects, and 3D models can be helpful for further studies of efficacy or cytotoxicity before using animal models [166]