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doi:10.1093/toxsci/kfm268 Hofmann AF

NAFLD could be considered an hepatic manifestation of metabolic syndrome, with a strong association with obesity, type 2 diabetes, hypertension, and dyslipidaemia (3)

To obtain serum, the blood was then allowed to clot for 10 minutes at room temperature before being centrifuged for 15 minutes at 3500 rpm using a SIGMA SM7000 centrifuge (SIGMA, UK)